In this article we will discuss about the tests for estimation of cellulase in plants. Principle: Endo-β-1, 4 glucanase acts on carboxymethyl cellulose (CMC) and hydrolyses the β-1, 4 glycosidic bonds in a random manner. As a result, the viscosity of CMC solution is reduced. This is measured in an Ostwald viscometer. Materials: a. Citrate-phosphate Buffer 0.1 M (pH 6.0) [...]
In this article we will discuss about the complexometric method for estimation of calcium and magnesium in plants. Principle: Many complex-forming ions or molecules lead to a stepwise formation of complexes. Each complex is characterized by its own stability constant. This property of stepwise formation of complex can be used in titrimetry and, thus, one can be able to estimate [...]
In this article we will discuss about the colorimetric method for estimation of amino acids in plants. Principle: Amino acid content can be estimated quantitatively by Colorimetric method using ninhydrin reagent. The reaction of ninhydrin (triketohydrindene hydrate) with amino acids is of particular importance in the regard. Ninhydrin is a powerful oxidizing agent and elicits the oxidative deamination of the [...]