In this article we will discuss about the tests for estimation of lipase in plants. Principle: The quantity of fatty acid released in unit time is measured by the quantity of NAOH required to maintain pH constant. The milliequivalent of alkali consumed is taken as a measure of the activity of the enzyme. Materials: a. Substrate: Take 2 ml of [...]
In this article we will discuss about the Kjeldahl method for the estimation of nitrogen in plants. Principle: Digestion of nitrogenous compounds by concentrated sulphuric acid in presence of catalysts (reduced iron powder, and a mixture of CuSO4 and K2SO4) produces ammonium compound. Further, such ammonium compound when distilled by steam in presence of strong alkali (40% NaOH soln.) produces [...]
In this article you will discuss about the tests for estimation of catalase in plants. Principle: The enzyme catalase is responsible for catalysing the decomposition of H2O into H2O and O2. The assay of enzyme depends on estimation of residual H2O2 by titration with KMnO4. Requirements: (a) Reagents: 1. H2O2 —0.2 volume 2. H2SO4—10% 3. 0.05 NKMnO4 solution. (b) Glass-wares: [...]