The determination of the base sequence of a nucleic acid is much more difficult than the determination of the sequence of amino acids of a protein, because there are only 4 types of monomers instead of 20. This explains why one began with the determination of the sequences of transfer ribonucleic acids (more than 200 sequences are known); these do [...]
The determination of the base sequence of a nucleic acid is much more difficult than the determination of the sequence of amino acids of a protein, because there are only 4 types of monomers instead of 20. This explains why one began with the determination of the sequences of transfer ribonucleic acids (more than 200 sequences are known); these do [...]
The techniques used to study molecular biology of nucleic acids are:- 1. Gel electrophoresis 2. Use of restriction endonucleases 3. DNA hybridization 4. Hybridization probes 5. Isolation of specific segments of DNA 6. DNA cloning 7. Cloning DNA in plasmid vectors 8. Vector DNA can be introduced into host organisms by transformation 9. Libraries of DNA molecules can be created [...]