Amino acids will be incorporated into proteins from the aminoacyl-tRNAs. To study this process we must acquire a wider knowledge of ribosomes and the messenger RNA, which will form a complex with the aminoacyl-tRNAs. Ribosomes: These are ribonucleoprotein particles consisting of about 65% RNAs and 35% proteins in the case of E.coli and which may be obtained from cellular homogenates [...]
In this article we will discuss about the physico-chemical properties of nucleic acids. The size of nucleic acids varies immensely. The smallest ribonucleic acids are the tRNAs which comprise about 80 nucleotides; their molecular weight is about 30 000. Ribosomal ribonucleic acids are larger; they contain several thousands of nucleotides and their molecular weight can exceed 1 million. The RNA [...]
The replication of numerous procaryotes was studied in recent years. The mechanisms of the replication of phages T4, T7 and λ are now well known, while those of B. subtilis are beginning to be understood. However, the most extensively studied system remains Escherichia coli, thanks to the combination of various types of approaches. Experimental Approaches to the Study of Replication: [...]