One of the most attractive features of light microscopy is the ease with which most specimens can be prepared for examination. Preparation often involves nothing more than mounting a small piece of the specimen in a suitable liquid on a glass slide and covering it with a glass coverslip. The slide is then positioned on the specimen stage of the [...]
Centrifugation is a useful means of isolating and purifying cellular components because most organelles and macromolecules differ from one another significantly in size and density (and sometimes also in shape, especially in the case of macromolecules). These properties, as well as the density and viscosity of the surrounding medium, determine the rate at which a specific particle will move when [...]
In this article we will discuss about the design of transmission electron microscope, explained with the help of a diagram. In TEM a finely focused beam of electrons from an electron gun is passed through a specially prepared ultra thin section of the specimen. The beam is focused on a small area of the specimen by an electromagnetic condenser lens. [...]