The following points highlight the top three staining methods used for colouring the biological materials. The types are: 1. Negative Staining 2. Simple Staining 3. Differential Staining. Method # 1. Negative Staining: For quicker observation of the morphology of bacterial or yeast cells, the negative staining techÂnique is applied. Requirements: 1. Bacterial/yeast suspension. 2. Slide, inoculating needle, pipettes etc. 3. [...]
In this article we will discuss about the Isolation of Various Microorganisms:- 1. Isolation of Algae, Fungi, Bacteria and Actinomycetes from Soil 2. Isolation of Bacteria from Milk Samples by Agar Plate Method 3. Isolation of Microorganisms from Air by Exposure Plate Technique 4. Isolation of Microorganisms from Water or Waste Water Samples 5. Isolation of Root Nodule Bacteria. Contents: [...]
The following points highlight the five main steps for culture of microorganisms. The steps are: 1. Preparation of Media 2. Adjustment of pH of Media 3. Preparation of Stabs and Slants 4. Pouring of Plates 5. Inoculation of Bacteria in Nutrient Slants and Agar Plates. Step # 1. Preparation of Media: Principle: In preparing a culture medium for any microorganism, [...]