The following points highlight the nine main types of culture of microorganisms. The types are: 1. Streak Culture 2. Stab or Stick Culture 3. Stock Cultures 4. Starter Culture 5. Enrichment Culture 6. Batch Culture 7. Continuous Culture 8. Synchronous Culture 9. Fed-Batch Culture. Type # 1. Streak Culture: Streak culture (Fig. 16.1) of a microorganism is that which has [...]
The following points highlight the top four methods used for maintenance and preservation of pure cultures. The methods are: 1. Refrigeration 2. Paraffin Method 3. Cryopreservation 4. Lyophilisation. Method # 1. Refrigeration: Pure cultures can be successfully stored at 0-4°C either in refrigerators or in cold-rooms. This method is applied for short duration (2-3 weeks for bacteria and 3-4 months [...]
The following points highlight the five main steps for culture of microorganisms. The steps are: 1. Preparation of Media 2. Adjustment of pH of Media 3. Preparation of Stabs and Slants 4. Pouring of Plates 5. Inoculation of Bacteria in Nutrient Slants and Agar Plates. Step # 1. Preparation of Media: Principle: In preparing a culture medium for any microorganism, [...]