The following points highlight the seven steps for identification of bacteria isolated from a specimen. The steps are: 1. Morphology and Staining 2. Cultural Characteristics 3. Biochemical Reactions 4. Antigenic Characters 5. Typing of Bacteria: Bacteriophage Sensitivity 6. Animal Pathogen City 7. Antibiotic Sensitivity. Identification of Bacteria: Step # 1. Morphology and Staining: Serve as preliminary criteria. The Gram stained [...]
In this article we will discuss about the Antibiotic Sensitivity Test of Bacteria. Subcultures of the test organisms are made in broth and incubated for 6-18 hours. Any density of inoculum may be used but in most cases the surfaces of cultures plates may be sown with broth containing approximately 100 million cells per ml. After drying the surface, the [...]
In this article we will discuss about Microorganisms:- 1. History of Microorganisms 2. Inhibition of Microorganisms: Antimicrobial Agents 3. Summary 4. Classes of Antibiotics. History of Microorganisms: When it was assumed that the communicable diseases were caused by microorganisms, the methods of sterilisation, disinfection and sanitation were adopted in the daily practice. In early nineteenth century, the hospitals were dirty [...]