The following points highlight the top six methods used for obtaining pure culture of microorganisms. The methods are: 1. Streak Plate Method 2. Pour Plate Method 3. Spread Plate Method 4. Serial Dilution Method 5. Single Cell Isolation Methods 6. Enrichment Culture Method. 1. Streak Plate Method: This method is used most commonly to isolate pure cultures of bacteria. A [...]
The following points highlight the top four types of culture media used in the cultivation of anaerobic bacteria. The culture medium are: 1. Special Anaerobic Culture Media 2. Anaerobic Chamber 3. Anaerobic Bags or Pouches 4. Anaerobic Jars. Type # 1. Special Anaerobic Culture Media (Prereduced Media): Of all the methods available for the cultivation of anaerobic bacteria, exclusion of [...]
In this article we will discuss about the nutritional requirements of microorganisms. Mineral Nutrients: The microbial nutrients can be classified as macro (major) nutrients, and micro (minor) nutrients or trace elements on the basis of their amount required. 1. Macro or Major Mineral Nutrients: The microbial cells contain water accounting for some 80-90% of their total weight and, therefore, the [...]