The following points highlight the six common laboratory instruments used in the culture of microorganisms. The instruments are: 1. Class-Wares 2. Colony Counter 3. Inoculation Chamber 4. Incubator 5. pH Meter 6. Nephelometer. Laboratory Instrument # 1. Class-Wares: 1. Culture tubes: Common test tubes in which are used corning or Pyrex glass tubes, are recommended because they have to overcome [...]
In this article we will discuss about the process of inoculation in microorganisms. Preparation of Agar Slant: Agar slants are-prepared (Fig. 16.11) to inoculate microbial culture. To prepare agar slant a required number of culture tubes are taken and about 12 to 15 ml of liquified agar medium is poured in each of them. The tubes are now cotton-plugged and [...]
The following points highlight the top four methods used for maintenance and preservation of pure cultures. The methods are: 1. Refrigeration 2. Paraffin Method 3. Cryopreservation 4. Lyophilisation. Method # 1. Refrigeration: Pure cultures can be successfully stored at 0-4°C either in refrigerators or in cold-rooms. This method is applied for short duration (2-3 weeks for bacteria and 3-4 months [...]