The following points highlight the six important methods of tissue culture. The methods are: 1. Hanging Drop Culture 2. Double Cover Slip Method 3. Culture in Stewards Auxophyton 4. Culture in Bottles, Conical Flasks etc. 5. Culture in a Micro-Chamber 6. Culture in Petridish, Watch Glass etc.. Method # 1. Hanging Drop Culture: The simplest method of tissue culture is [...]
The following methods highlight the two important methods of gene transfer used in plants. The methods are: 1. Indirect Method 2. Direct Method. 1. Indirect Method: Transfer via Agrobacterium: The transforÂmation of a plant can be carried out directly by using Agrobacterium spp. which is a common bacterium causing crown gall tumour in legumes. This bacterium carries a plasmid with [...]
The following points highlight the top three methods of recombinant DNA formation. The methods are: 1. Transformation 2. Transfection 3. Non-Bacterial Transformation. Recombinant DNA Formation: Method # 1. Transformation: The restriction enzyme which causes a break in foreign DNA also causes a staggered cut in the vector DNA at a specific cleavage site. The cohesive ends of vector DNA possess [...]