The following points highlight the top six methods that are used for preservation of organisms. The methods are: 1. Agar Slant Culture 2. Agar Slant Culture Covered with Oil 3. Saline Suspension 4. Preservation at Very Low Temperature 5. Preservation by Drying in Vacuum 6. Lyophilization or Freeze Drying. Method # 1. Agar Slant Culture: Agar slants are prepared in [...]
The following points highlight the three methods for culture maintenance which seems to be generally used in the fermentation industries: 1. Preservation of Culture by Drying 2. Maintenance of Cultures by Storage with Limited Metabolic Activity 3. Preservation by Lyophilization. Method # 1. Preservation of Culture by Drying: These are different methods of drying of cultures given below: (a) Dried [...]
In this article we will discuss about the improvement of microbial strains for better production of products. 1. Methods of Strain Improvement: A mutant requiring oleic acid for neomycin formation by Streptomyces fradiae showed a decrease in the intracellular level of neomycin precursors in the mutant. On the other hand supersensitive mutants of β-lactam antibiotics are another example. Recent approaches [...]