Geneticists use following two methods for sequencing the genomes: 1. Clone-by-clone Method 2. Shotgun Cloning. Method # 1. Clone-by-Clone Method: This method was the first to be developed. It begins with the construction of genomic libraries of restriction fragments covering all the genomic DNA (or genomic clones) of an organism. By using genetic markers, overlapping clones are assembled into genetic [...]
In this article we will discuss about the influence of PCR on DHPLC polymorphism characterization. Introduction: Denaturing high performance liquid chromatography (DHPLC) has emerged as a powerful tool to detect polymorphisms and mutations in genomes of living organisms. Relative to other mutation detection techniques such as sequencing, primer extension, and SSCP, DHPLC is: 1. Sensitive: Clear separation between signal and [...]
In this article we will discuss about the significance of genomics in agriculture. The main goal of agriculture research is to improve of economically important crops like rice, wheat, corn, oilseeds and vegetables etc. For the production of high quality food in substantial quantity, the above goal can be accomplished by optimising plant growth overcoming abiotic and biotic stress. Therefore, [...]