In this article we will discuss about the mode of action of antisense RNA. Several possible mechanisms are involved during antisense inhibition of specific gene. Following are some of the most promising mode of actions have been suggested (Fig. 22.3). Action in the Nucleus Premise: One of the earliest methods in antisense inhibition is carried out by blocking transcription. This [...]
Molecular markers are DNA sequences, whose inheritance pattern can be established. Classic examples of molecular markers are: 1. Restriction Fragment Length Polymorphism (RFLP) 2. Randomly Amplified Polymorphic DNA (RAPD) 3. Amplified Fragments Length Polymorphism (AFLP) 4. Sequence Characterized Amplified Region (SCAR). Example # 1. Restriction Fragment Length Polymorphism (RFLP): This novel non-PCR based approach is based on hybridization of probe [...]
In this article we will discuss about the manipulation of fruit softening by antisense RNA. Plant cell wall is responsible for providing mechanical strength to plant organs. Tissue and cells due to their complex structure and biomolecules are involved. During fruit ripening several hydrolytic enzymes such as PG, PME and cellulase cleaves various polymers that constitute cell wall and consequently [...]