In this article we will discuss about the restriction maps and RFLPS. Also learn about the significance of restriction maps. The number of cuts that a restriction enzyme makes in a segment of double-stranded DNA depends on the size of that DNA, its sequence, and the number of base pairs in the recognition sequence of the particular enzyme. That is, [...]
The following points highlight the six significant techniques of gene transfer by help of agrobacterium tumefaciens. The techniques are: 1. Regeneration of Plants from Protoplasts and Callus Tissues 2. The Ti plasmid of Agrobacterium Tumefaciens 3. Formation of Non-oncogenic (or Disarmed) Ti Plasmids 4. Chimeric Selectable Marker Genes and a few others. Technique # 1. Regeneration of Plants from Protoplasts [...]
This essay deals with the restriction enzymes and other useful enzymes which are commonly used in genetic engineering. Enzymes Used in Genetic Engineering Essay Contents: Essay on the Discovery of Enzymes Essay on Restriction Endonuclease Enzyme Essay on Ligase Enzymes Essay on Alkaline Phosphatase Enzyme Essay on Phosphonucleotide Kinase Enzyme Essay on S1 Nuclease Enzyme Essay on DNA Polymerase I: [...]