The following points highlight the top two methods of gene transfer. The methods are: 1. Indirect Method (Agrobacterium Mediated Gene Transfer) 2. Direct Methods of Gene Transfer. Method # 1. Indirect Method (Agrobacterium Mediated Gene Transfer): The transformation of a plant can be carried out directly by using Agrobacterium spp. which is a common bacterium causing crown gall tumour in [...]
In this article we will discuss about the methods of gene transfer. Gene transfer can be done by two methods: (i) Indirect Method through Vectors or Carriers and (ii) Direct or Vectorless Transfer Method. The first method includes: (a) Plasmids and Viruses and (b) Natural Genetic Engineer, (a) Plasmids and Viruses: Recombinant DNA technology involves the transfer of desired genes [...]
The following points highlight the three modes of gene transfer and genetic recombination in bacteria. The modes are: 1. Transformation 2. Transduction 3. Bacterial Conjugation. Mode # 1. Transformation: Historically, the discovery of transformation in bacteria preceded the other two modes of gene transfer. The experiments conducted by Frederick Griffith in 1928 indicated for the first time that a gene-controlled [...]