Tag Archives | Gene Transfer

Methods of Gene Transfer used in Plants

The following methods highlight the two important methods of gene transfer used in plants. The methods are: 1. Indirect Method 2. Direct Method. 1. Indirect Method: Transfer via Agrobacterium: The transfor­mation of a plant can be carried out directly by using Agrobacterium spp. which is a common bacterium causing crown gall tumour in legumes. This bacterium carries a plasmid with [...]

By |2016-07-14T18:34:53+00:00July 14, 2016|Genetic Engineering|Comments Off on Methods of Gene Transfer used in Plants
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3 Main Ways for Confirming Gene Transfer

The following points highlight the three main ways for confirming gene transfer. The ways are: 1. Marker and Reporter Gene 2. Southern Blotting or Southern Hybri­dization 3. Northern Hybridization. Way # 1.Marker and Reporter Gene: Transfor­mation in eukaryotic system is a very low fre­quency event. It is, therefore, important that there should be some means for selecting the trans­formed plant [...]

By |2016-07-14T18:34:48+00:00July 14, 2016|Genetic Engineering|Comments Off on 3 Main Ways for Confirming Gene Transfer
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