The length of DNA double helix is calculated in a typical mammalian cell (simply by multiplying the total number of bp with distance between two consecutive bp, that is, 6.6 x 109 bp x 0.34 x 10-9m/bp), which approximately comes out to be 2.2 meters. A length that is far greater than the dimension of a typical nucleus (approximately 10-6 [...]
Recombinant DNA (rDNA) technology involves the following six stages: 1. Isolation of the Genetic Material (DNA) 2. Cutting of DNA at Specific Locations 3. Amplification of Gene of Interest Using PCR 4. Preparation and Insertion of Recombinant DNA into the Host Cell/Organism 5. Obtaining the Foreign Gene Product! 1. Isolation of the Genetic Material (DNA): Nucleic acid (DNA or RNA) [...]
This article provides useful notes on the Biosynthesis of DNA Replication! The biosynthesis of deoxyribonucleic acid (DNA) in vitro through polymerization of nucleoside triphosphates in the presence of the appropriate enzyme was first successfully completed by Kornberg, and for this he received the Nobel Prize in 1959. The nucleic acid DNA consists of four kinds of nucleotides: units of purine [...]
