Tag Archives | Cloning of Genes

Cloning of Genes: Methods and Advantages | Biochemistry

In this article we will discuss about: 1. Methods of Cloning, 2. Cloning of a Specific Gene, 3. Cloning of a Specific Gene, 4. Expression of Cloned Genes, 5. Cloning of Genes in Eucaryotic Cells and  6. Precautions to be taken during Experiments of Genetic Recombination. Methods of Cloning: In the past few years, an increasing number of laboratories have [...]

By |2016-12-23T06:26:26+00:00December 23, 2016|Genetics|Comments Off on Cloning of Genes: Methods and Advantages | Biochemistry
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Cloning of Specific Genes and their Identification

In this article we will discuss about the top five techniques used for cloning of specific genes and their identification. The techniques are: 1. Direct Selection of Recombinant Clones 2. Colony Hybridization 3. Complementary DNA (c-DNA) 4. Isolation of a Specific Gene: Southern Blotting 5. Polymerase Chain Reaction (PCR). The restriction digest always contains an assortment of donor DNA fragments [...]

By |2016-09-01T09:13:21+00:00September 1, 2016|Recombinant DNA Technology|Comments Off on Cloning of Specific Genes and their Identification
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