In this article we will discuss about: 1. Methods of Cloning, 2. Cloning of a Specific Gene, 3. Cloning of a Specific Gene, 4. Expression of Cloned Genes, 5. Cloning of Genes in Eucaryotic Cells and 6. Precautions to be taken during Experiments of Genetic Recombination. Methods of Cloning: In the past few years, an increasing number of laboratories have [...]
In this article we will discuss about plant and animal cloning. In plants, agronomically important traits are governed by the genetic information stored in the nuclear and organellar (i.e., chloroplasts and mitochondria) genome and gene transfer can be done in all these genomes. However, gene transfer in organelles specially in mitochondria is relatively difficult as compared to nuclear genetic transformation. [...]
The following points highlight the five main types of cloning. The types are: 1. Microbial Cloning 2. Gene Cloning 3. Cell Cloning 4. Plant Cloning 5. Organismal Cloning. Type # 1. Microbial Cloning: From very ancient times, it is known that a variety of microbes such as bacteria and yeast (fungus) are helpful in making a large number of products, [...]