In this article we will discuss about plant and animal cloning. In plants, agronomically important traits are governed by the genetic information stored in the nuclear and organellar (i.e., chloroplasts and mitochondria) genome and gene transfer can be done in all these genomes. However, gene transfer in organelles specially in mitochondria is relatively difficult as compared to nuclear genetic transformation. [...]
The following points highlight the five main types of cloning. The types are: 1. Microbial Cloning 2. Gene Cloning 3. Cell Cloning 4. Plant Cloning 5. Organismal Cloning. Type # 1. Microbial Cloning: From very ancient times, it is known that a variety of microbes such as bacteria and yeast (fungus) are helpful in making a large number of products, [...]
Read this article to learn about the cloning vectors and the factors considered for the treatment of cloning vector system. The seven factors considered for the treatment of cloning vector system are: (1) Plasmids (2) Bacteriophage I as a Cloning Vector (3) Cosmids (4) Fosmids (5) Phagemid (6) Shuttle Vectors and (7) Artificial Chromosomes. Cloning Vectors: For the cloning of [...]