Object: To separate the chlorophyll pigments by Thin Layer Chromatography (T.L.C.). Requirements: Spinach leaves, slides, staining tube, acetone, benzene, mortar, pastel, Kieselgel G, drier, pencil, sand, distilled water and oven. Preparation of T.L.C. Slides: 1. Prepare a slurry of Kieselgel by suspending about 2 gm. of Kieselgel in 10 ml. of distilled water. 2. Take 4 clean microscopic slides and [...]
The below mentioned article includes a list of three experiments on paper chromatography. 1. Experiment to separate amino acids in a mixture by paper strip chromatography: Principle: It is based on the fact that paper chromatography separates compounds on the basis of their different rates of migration of filter paper (cellulose). The rates of migration depend upon the solvent which [...]
The following points highlight the six full proof techniques of chromatography. The techniques are: 1. Partition Chromatography 2. Ion Exchange Chromatography 3. Adsorption Chromatography 4. Gel Permeation Chromatography 5. Gas Chromatography Principle 6. High Performance Liquid Chromatography (HPLC). Technique # 1. Partition Chromatography: Two immiscible liquid phases are passed through the supporting phase-containing materials. This is also called Liquid-Liquid Chromatography. [...]