Chromatography was originally introduced by Tswett in 1906, a Polish Botanist, for separation of different colour pigments present in the plant extract. Amino acids can also be separated from one another by partition chromatography. Chromatography might be of different types, such as: (a) Paper, (b) Thin-layer, (c) Column, and The amino acids are separated between a stationary and a mobile [...]
The following points highlight the three main types of chromatography. The types are: 1. Adsorption Column Chromatography 2. Thin Layer Chromatography 3. Gas Chromatography. Type # 1. Adsorption Column Chromatography: The general chromatography method was introduced into biology by Tswett in 1906 and various adsorbents such as starch and aluminium oxide are poured as a slurry into a long glass [...]
Object: To separate leaf pigments by column chromatography. or To separate the chloroplast pigments by solvent method. Requirements: Pigments extract, magnesium oxide, filter glass disc, glass wool, flask, suction pump, chromatographic tube, petroleum ether, benzene. Method: 1. Take a chromatographic tube having a filter glass disc and glass wool at its base. 2. Fill the chromatographic tube with magnesium oxide. [...]