Chromatography was originally introduced by Tswett in 1906, a Polish Botanist, for separation of different colour pigments present in the plant extract. Amino acids can also be separated from one another by partition chromatography. Chromatography might be of different types, such as: (a) Paper, (b) Thin-layer, (c) Column, and The amino acids are separated between a stationary and a mobile [...]
The following points highlight the three main types of chromatography. The types are: 1. Adsorption Column Chromatography 2. Thin Layer Chromatography 3. Gas Chromatography. Type # 1. Adsorption Column Chromatography: The general chromatography method was introduced into biology by Tswett in 1906 and various adsorbents such as starch and aluminium oxide are poured as a slurry into a long glass [...]
Object: To separate the chlorophyll pigments by Thin Layer Chromatography (T.L.C.). Requirements: Spinach leaves, slides, staining tube, acetone, benzene, mortar, pastel, Kieselgel G, drier, pencil, sand, distilled water and oven. Preparation of T.L.C. Slides: 1. Prepare a slurry of Kieselgel by suspending about 2 gm. of Kieselgel in 10 ml. of distilled water. 2. Take 4 clean microscopic slides and [...]