The following points highlight the three main types of chromatography. The types are: 1. Adsorption Column Chromatography 2. Thin Layer Chromatography 3. Gas Chromatography. Type # 1. Adsorption Column Chromatography: The general chromatography method was introduced into biology by Tswett in 1906 and various adsorbents such as starch and aluminium oxide are poured as a slurry into a long glass [...]
The following points highlight the six full proof techniques of chromatography. The techniques are: 1. Partition Chromatography 2. Ion Exchange Chromatography 3. Adsorption Chromatography 4. Gel Permeation Chromatography 5. Gas Chromatography Principle 6. High Performance Liquid Chromatography (HPLC). Technique # 1. Partition Chromatography: Two immiscible liquid phases are passed through the supporting phase-containing materials. This is also called Liquid-Liquid Chromatography. [...]
Let us learn about Thin Layer Chromatography. Introduction to Thin Layer Chromatography: Chromatography is used to separate mixtures of substances into their components. All forms of chromatography work on the same principle. They all have a stationary phase (a solid, or a liquid supported on a solid) and a mobile phase (a liquid or a gas). The mobile phase flows [...]