The below mentioned article provides a note on cybrid production. In sexual hybridization process the nuclear fusion takes place between two parents, but cytoplasm is contributed by the maternal parent only. Whereas in case of somatic hybrids due to cell fusion the nucleus as well as cytoplasm derived from both the parents. But in the fusion programme it is possible [...]
The following points highlight the top eight practical applications of protoplast culture. Application # 1. Production of Somatic Hybrids: To overcome the sexual incompatibility barriers the protoplast fusion programme may be taken to obtain the interspecific and inter-generic hybrids. For example, the fusion between Lycopersicon esculentum (tomato) and Solanum tuberosum (potato) have been possible to obtain the somatic hybrid 'Pomato', [...]
In this article we will discuss about:- 1. Method of Protoplast Fusion 2. Mechanism of Protoplast Fusion 3. Identification and Selection 4. Verification and Characterisation. Isolated protoplasts are devoid of cell walls which make them easy tools for underÂgoing fusions in vitro. An important factor is that generally there is incompatibility barrier between two protoplasts of different species or genera. [...]