In this article we will discuss about the procedure of embryo culture. Source Material: Physiologically uniform embryos of the same size and same growth phase should be used for culture, which can be achieved if the plants are raised and maintained under controlled condition. For supply of materials regularly the selected plants should bear the flowers at regular intervals; to [...]
In this article we will discuss about:- 1. Sources of Explant for Protoplast Isolation 2. Methods of Protoplast Isolation 3. Purification of Protoplasts 4. Isolation of Sub-Protoplasts 5. Protoplast Viability Test. Each plant cell has a definite cellulose wall which is attached with each other by middle lamella made up of pectin. Protoplast is the content which is surrounded only [...]
In this article we will discuss about the stages and methods of protoplast culture. Isolated protoplasts are cultured either in a liquid medium or semisolid agar medium in a thin layer or as small drops of nutrient medium in petridish. The medium for protoplast culture requires the same component as required for callus or suspension culture. Increasing the calcium concentration [...]