The below mentioned article provides a study note on organogenesis. In plant tissue culture, organogenesis is a process of differentiation by which plant organs like roots, shoots, buds etc. are formed from the unusual points of origin of an organized explants where a preformed meristem is lacking. Plant development through organogenesis is the formation of organs either de novo or [...]
In this article we will discuss about:- 1. Sources of Explant for Protoplast Isolation 2. Methods of Protoplast Isolation 3. Purification of Protoplasts 4. Isolation of Sub-Protoplasts 5. Protoplast Viability Test. Each plant cell has a definite cellulose wall which is attached with each other by middle lamella made up of pectin. Protoplast is the content which is surrounded only [...]
In this article we will discuss about the stages and methods of protoplast culture. Isolated protoplasts are cultured either in a liquid medium or semisolid agar medium in a thin layer or as small drops of nutrient medium in petridish. The medium for protoplast culture requires the same component as required for callus or suspension culture. Increasing the calcium concentration [...]