In this article we will discuss about the procedure of embryo culture. Source Material: Physiologically uniform embryos of the same size and same growth phase should be used for culture, which can be achieved if the plants are raised and maintained under controlled condition. For supply of materials regularly the selected plants should bear the flowers at regular intervals; to [...]
In this article we will discuss about the stages and methods of protoplast culture. Isolated protoplasts are cultured either in a liquid medium or semisolid agar medium in a thin layer or as small drops of nutrient medium in petridish. The medium for protoplast culture requires the same component as required for callus or suspension culture. Increasing the calcium concentration [...]
The below mentioned article provides a note on cybrid production. In sexual hybridization process the nuclear fusion takes place between two parents, but cytoplasm is contributed by the maternal parent only. Whereas in case of somatic hybrids due to cell fusion the nucleus as well as cytoplasm derived from both the parents. But in the fusion programme it is possible [...]