The synthesis of nucleic acids (DNAs and RNAs) from nucleosides-5'- triphosphates is characterized by the necessity of conserving the genetic information intact. The DNA is indeed the carrier of this information which is transmitted from generation to generation; this notion is now well established, but it was long believed that this information was carried by proteins. It would therefore be [...]
In this article we will discuss about: 1. Methods of Cloning, 2. Cloning of a Specific Gene, 3. Cloning of a Specific Gene, 4. Expression of Cloned Genes, 5. Cloning of Genes in Eucaryotic Cells and 6. Precautions to be taken during Experiments of Genetic Recombination. Methods of Cloning: In the past few years, an increasing number of laboratories have [...]
In this article we will discuss about the process of metabolism in amino acids. We know that ammonia can be formed and then be found in the form of amino group of glutamic acid. We have studied the reactions — transamination and deamination — by which amino groups can be added to or removed from amino acids. The forms in [...]