Archive | Proteins

Molecular Biology of Proteins: 5 Techniques | Microbiology

The important techniques involved in the study of molecular biology of proteins are:- 1. Purification from Cell Extracts 2. Using Column Chromatography 3. Affinity Chromatography 4. Separation of Proteins on Polyacrylamide Gels 5. Antibodies can be Visualized Electrophoretically: Separated Proteins 6. Sequencing Protein Molecules. Technique # 1. Purification from Cell Extracts: Purification of proteins is a major part of understanding [...]

By |2016-12-20T11:20:17+00:00December 20, 2016|Proteins|Comments Off on Molecular Biology of Proteins: 5 Techniques | Microbiology
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Proteins (Exam Questions and Answers) | Microbiology

Everything you need to know about proteins. Some of the most frequently asked questions are as follows:- Q.1. What are proteins? Ans: A protein is a macromolecule composed of one or more polypeptide chains, each with a characteristic sequence of amino acids linked by peptide bonds. The cells of organisms contain thousands of different proteins each with a different function [...]

By |2016-12-20T11:20:15+00:00December 20, 2016|Proteins|Comments Off on Proteins (Exam Questions and Answers) | Microbiology
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Translation and Protein Biosynthesis | Genetics

A mRNA may be associated with several ribosomes (polysomes) in a cell actively engaged in protein synthesis. The process takes place in 4 major steps namely activation of amino acids, chain initiation, elongation and termination. Each step is controlled by specific enzymes and cofactors. Step # 1. Activation of Amino Acids: The amino acids are activated by a class of [...]

By |2016-12-12T07:24:37+00:00December 12, 2016|Proteins|Comments Off on Translation and Protein Biosynthesis | Genetics
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