In this article we will discuss about the role of auxins and cytokinins in culture medium. Effective, speedy growth and desirable morphogenesis in tissue culture requires the presence of both auxins and cytokinins in culture medium. The relative concentration of auxins and cytokinins are often essential to bring about some kind of morphogenesis. Axillary shoot proliferation in some species may [...]
In this article we will discuss about the three main plant hormone signalling involved in differential development and other physiological process in plants:- 1. Cytokinin Signalling 2. Auxin Signalling 3. Gibberellin Signalling. 1. Cytokinin Signalling: Cytokininis have been registered as one of the key plant hormones involved in differential development and other physiological process in plants, such as cell division, [...]
Generally, isolation of protoplast is categorized into mechanical and enzymatic. Also learn about the techniques involved in the isolation of leaf mesophyll protoplast. Mechanical Method: This method is crude and oldest method for protoplast isolation. It is only an historical perspective. It involves chopping and plasmolysis of tissues. Time consuming and low yield of protoplast are the main constraints in [...]