In this article we will discuss about the procedure of embryo culture. Source Material: Physiologically uniform embryos of the same size and same growth phase should be used for culture, which can be achieved if the plants are raised and maintained under controlled condition. For supply of materials regularly the selected plants should bear the flowers at regular intervals; to [...]
The below mentioned article provides a note on anther culture. Explants used for anther culture is very critical as the anther lobes bearing the PMC (pollen mother cell) of correct divisional stage signify for being divided to form the callus mass or the direct haploid embryo. Young flower buds with immature anther lobes are surface sterilised and the stamens are [...]
In this article we will discuss about the meaning and advantages of pollen culture. Meaning of Pollen Culture: Microspore or the immature pollen can be used as the explant to get the haploid plants directly. For pollen or microspore culture the flower buds are collected, surface sterilized and the anther lobes are dissected out from the flower buds as before. [...]