Natural occurrence of antisense RNA and its role in gene regulation have been realised long ago. Gene expression in both prokaryotes and eukaryotes are controlled by the product of regulatory gene, which are supposed to be proteins only. Recently, naturally occurring regulatory gene that directs the synthesis of RNA which can control gene expression. This newly discovered RNA repressor can [...]
Realising the role of antisense RNA technology in the reduction of ethylene production, a cDNA (pTOM13) encoding ACC oxidase has been isolated from a tomato fruit ripening library. An antisense gene construct from the cDNA strongly inhibited expression of ACC oxidase activity in transgenic tomatoes. A chimaeric pTOM13 antisense gene, encodes ACC oxidase consisting of 1.3 kilobase (kb) fragment of [...]
The following points highlight the two main types of antisense methods that can be employed to inhibit the expression of a target gene. These antisense methods are antisense oligonucleotides and antisense RNA. All these methods are basically designed to blockade or degradation of a specific target mRNA as a consequence of binding of nucleic acids complementary to sequence of the [...]