Archive | Plant Biotechnology

Naturally Occurring of Antisense RNA | Plants

Natural occurrence of antisense RNA and its role in gene regulation have been realised long ago. Gene expression in both prokaryotes and eukaryotes are controlled by the product of regulatory gene, which are supposed to be proteins only. Recently, naturally occurring regulatory gene that directs the synthesis of RNA which can control gene expression. This newly discovered RNA repressor can [...]

By |2016-12-23T06:34:52+00:00December 23, 2016|Plant Biotechnology|Comments Off on Naturally Occurring of Antisense RNA | Plants

Strategies for Ethylene Reduction | Plants

Realising the role of antisense RNA technology in the reduction of ethylene production, a cDNA (pTOM13) encoding ACC oxidase has been isolated from a tomato fruit ripening library. An antisense gene construct from the cDNA strongly inhibited expression of ACC oxidase activ­ity in transgenic tomatoes. A chimaeric pTOM13 antisense gene, encodes ACC oxidase consist­ing of 1.3 kilobase (kb) fragment of [...]

By |2016-12-23T06:34:52+00:00December 23, 2016|Plant Biotechnology|Comments Off on Strategies for Ethylene Reduction | Plants

Types of Antisense Methods | Plants

The following points highlight the two main types of antisense methods that can be employed to inhibit the expression of a target gene. These antisense methods are antisense oligonucleotides and antisense RNA. All these methods are basically designed to blockade or degradation of a specific target mRNA as a consequence of binding of nucleic acids complemen­tary to sequence of the [...]

By |2016-12-23T06:34:52+00:00December 23, 2016|Plant Biotechnology|Comments Off on Types of Antisense Methods | Plants
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