Realising the role of antisense RNA technology in the reduction of ethylene production, a cDNA (pTOM13) encoding ACC oxidase has been isolated from a tomato fruit ripening library. An antisense gene construct from the cDNA strongly inhibited expression of ACC oxidase activity in transgenic tomatoes. A chimaeric pTOM13 antisense gene, encodes ACC oxidase consisting of 1.3 kilobase (kb) fragment of [...]
The following points highlight the two main types of antisense methods that can be employed to inhibit the expression of a target gene. These antisense methods are antisense oligonucleotides and antisense RNA. All these methods are basically designed to blockade or degradation of a specific target mRNA as a consequence of binding of nucleic acids complementary to sequence of the [...]
In this article we will discuss about the mode of action of antisense RNA. Several possible mechanisms are involved during antisense inhibition of specific gene. Following are some of the most promising mode of actions have been suggested (Fig. 22.3). Action in the Nucleus Premise: One of the earliest methods in antisense inhibition is carried out by blocking transcription. This [...]