The following points highlight the top four methods used for maintenance and preservation of pure cultures. The methods are: 1. Refrigeration 2. Paraffin Method 3. Cryopreservation 4. Lyophilisation. Method # 1. Refrigeration: Pure cultures can be successfully stored at 0-4°C either in refrigerators or in cold-rooms. This method is applied for short duration (2-3 weeks for bacteria and 3-4 months [...]
The following points highlight the top four types of culture media used in the cultivation of anaerobic bacteria. The culture medium are: 1. Special Anaerobic Culture Media 2. Anaerobic Chamber 3. Anaerobic Bags or Pouches 4. Anaerobic Jars. Type # 1. Special Anaerobic Culture Media (Prereduced Media): Of all the methods available for the cultivation of anaerobic bacteria, exclusion of [...]
In this article we will discuss about the nutritional requirements of microorganisms. Mineral Nutrients: The microbial nutrients can be classified as macro (major) nutrients, and micro (minor) nutrients or trace elements on the basis of their amount required. 1. Macro or Major Mineral Nutrients: The microbial cells contain water accounting for some 80-90% of their total weight and, therefore, the [...]