In this article we will discuss about the use of transduction of genetic mapping in viruses. Viruses are the very small particles, hence they are considered unsuitable to study inheritance. Because scorable traits are not available with them. However, some of the bacterial characters have been used for inheritance and recombination studies such as plaque morphology (large or small, fuzzy [...]
In this article we will discuss about the processing of pre-tRNA in E.coli and yeast. In prokaryotes, there are many operons (e.g. 7 genes in E. coli) which are separated by spacer sequences. Mature tRNA molecules are generated by processing longer pre-tRNA transcripts. Through a specific step, RNases D, E, F and P generate these mature tRNA molecules by exo-and [...]
In this article we will discuss about the processing of mRNA in prokaryotes and eukaryotes. In prokaryotes, there is a little or no processing of mRNA transcripts. Prokaryotic mRNA is degraded very rapidly from 5' end. Therefore, to rescue from degradation it is translated before being finally transcribed. Ribosomes assemble on unfinished mRNA and first cistron (protein coding region) can [...]