Archive | Genetics

Transformation Process of Plants | Genetics

Transformation of plants can be successfully accomplished by employing two basic strategies. One is co-cultivation of protoplast with Agrobacterium and the leaf disc procedure. The first transgene was obtained by co-cultivation. Basic methodology involves Agrobacterium transformation of regenerating protoplast, plus screening and regeneration. Leaf-disc transformation is used extensively in most of the transformation work. Surface- sterilized leaf disc or pieces [...]

By |2016-12-23T06:34:56+00:00December 23, 2016|Genetics|Comments Off on Transformation Process of Plants | Genetics
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CamV35S Promoter: Molecular Dissection and Functional Region | Genetics

In this article we will discuss about:- 1. Subject Matter of CamV35S Promoter 2. Molecular Dissection of CamV35S Promoter 3. Functional Regions. Subject Matter of CamV35S Promoter: The 35S promoter is one of the major promoters of cauliflower mosaic virus (CamV). This virus infects crucifers and contains double-stranded genome as the genetic material. Based on the 8 kb genetic map, [...]

By |2016-12-23T06:34:56+00:00December 23, 2016|Genetics|Comments Off on CamV35S Promoter: Molecular Dissection and Functional Region | Genetics
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Chemically Inducible Promoters and its Types | Genetics

Developments of promoters that are activated in response to a specific chemical are particularly desirable in plant transformation. Utility of chemically inducible promoters are increasingly relevant to the improvement of crops by genetic engineering. Besides, several biotechnological applications of chemically inducible promoters have been witnessed recently such as construction of a male sterile system, expression of transgene that interfere with [...]

By |2016-12-23T06:34:56+00:00December 23, 2016|Genetics|Comments Off on Chemically Inducible Promoters and its Types | Genetics
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