In this article we will discuss about:- 1. Single Transduction Pathways in Plants 2. Mechanism of Signal Transduction 3. Calcium Signalling in Plants 4.Other Plant Signalling Molecules Single Transduction Pathways in Plants: Plant cells, due to their sessile nature, are able to interact with its surrounding environÂment. Plants use various environmental signals to alter their mode of developmental morpholÂogy. Throughout [...]
Depending on the nature of selection, marker genes are divided into two types: 1. Selectable Marker Genes 2. Reporter Genes. Type # 1. Selectable Marker Genes: One of the constraints in plant transformation technique is the obtaining of transformed plants with less frequency. Thus, it is indispensable to select transformed cells under cultural conditions itself. Selection is based on the [...]
Transformation of plants can be successfully accomplished by employing two basic strategies. One is co-cultivation of protoplast with Agrobacterium and the leaf disc procedure. The first transgene was obtained by co-cultivation. Basic methodology involves Agrobacterium transformation of regenerating protoplast, plus screening and regeneration. Leaf-disc transformation is used extensively in most of the transformation work. Surface- sterilized leaf disc or pieces [...]