The following points highlight the three main ways for confirming gene transfer. The ways are: 1. Marker and Reporter Gene 2. Southern Blotting or Southern Hybridization 3. Northern Hybridization. Way # 1.Marker and Reporter Gene: Transformation in eukaryotic system is a very low frequency event. It is, therefore, important that there should be some means for selecting the transformed plant [...]
In this article we will discuss about the Controversies and Hazards of Genetic Engineering. Although recombinant DNA technology has played tremendous roles in many aspects, yet it may bring ecological catastrophs and disturb balance of nature. It is difficult to assess the nature's destruction in ones' lifetime. It is beyond man's control as it has vast transformational potential. Sometimes genetic [...]
The following points highlight the four steps of genetic engineering in plants. The steps are: 1. Isolation and Identification of Desired DNA/Genes 2. Cloning and Production of Identical Copies of Isolated DNA Segment 3. Introduction of Cloned DNA into Plant Cells and its Integration with Plant DNA 4. Expression of Introduced Genes in the Plants. Steps of Genetic Engineering in [...]