The following points highlight the three main ways for confirming gene transfer. The ways are: 1. Marker and Reporter Gene 2. Southern Blotting or Southern Hybridization 3. Northern Hybridization. Way # 1.Marker and Reporter Gene: Transformation in eukaryotic system is a very low frequency event. It is, therefore, important that there should be some means for selecting the transformed plant [...]
Subject Matter of Genetic Engineering: DNA is a genetic material which contains all hereditary information needed to create an organism. DNA actually does not make organism, it only makes proteins. DNA is transcribed into mRNA and mRNA is translated into protein and the protein then forms organism. The specific regions on the DNA molecule that direct the synthesis of proteins [...]
In this article we will discuss about the Controversies and Hazards of Genetic Engineering. Although recombinant DNA technology has played tremendous roles in many aspects, yet it may bring ecological catastrophs and disturb balance of nature. It is difficult to assess the nature's destruction in ones' lifetime. It is beyond man's control as it has vast transformational potential. Sometimes genetic [...]