The following points highlight the top two screening techniques used in genetic engineering:- 1. Colony Hybridisation Technique 2. Blotting Techniques. Screening Technique # 1. Colony Hybridisation Technique: This technique has been developed by Gaustein and Hogness (1975). It requires the radioactively labelled DNA probe with a sequence complementary to at least one part of the desired DNA. The probe may [...]
The following points highlight the top two types of molecular maps. The types are: 1. Molecular Physical Map 2. Molecular Genetic Map. Type # 1. Molecular Physical Map: In molecular physical map, actual distances are measured in terms of nucleotide sequences. (a) In Situ Hybridization (ISH): Fluorescence in situ hybridization (FISH) and Genomic in situ hybridization (GISH): The technique of [...]
The following points highlight the four main techniques used for gene cloning. The techniques are: 1. Isolation of DNA to be Cloned 2. Insertion of Foreign DNA Fragment into a Vector 3. Transfer of Recombinant DNA into Bacterial Cell 4. Detection of Recombinant Clone. Technique # 1. Isolation of DNA to be Cloned: The DNA of interest, i.e., target DNA [...]