Read this article to learn about the manipulation of gene expression in prokaryotic host cells and eukaryotic host cells. Selection of Host Cells for Gene Expression: The nature of a host cell or an organism is as important as the nature of a vector. The most important requirements of a good host include its suitable cultivation in the laboratory, besides [...]
Read this article to learn about the techniques used for isolating genes from any organism for synthesis of naturally occurring proteins. Site-Directed Mutagenesis: With the advances in genetic engineering, genes can be isolated (from any organism) and used for the synthesis of naturally occurring proteins. Some of these proteins serve as enzymes (i.e., biocatalysts), and a selected few of them [...]
This article throws light upon the six methods of gene transfer. The six methods are: (1) Transformation (2) Conjugation (3) Electroporation (4) Liposome-Mediated Gene Transfer (5) Transduction and (6) Direct Transfer of DNA. Method # 1. Transformation: Transformation is the method of introducing foreign DNA into bacterial cells (e.g. E.coli). The uptake of plasmid DNA by E.coli is carried out [...]