The following points highlight the top four techniques used for the isolation of microbial mutants. The techniques are: 1. Direct Observation 2. Enrichment Technique 3. Replica-Plating Technique 4. The Ames Test. 1. Direct Observation Technique: In some cases, a colony growing on an agar plate can easily be seen to be different from the normal parental type (wild-type). For example, [...]
Essay on Regulation of Gene Expression:- 1. Introduction to Regulation of Gene Expression 2. The Lac Operon 3. Bacteriophage Lambda (λ) 4. Gene Amplification during Development of Metazoans 5. Immunoglobulin Gene Rearrangement 6. Regulation of Messenger RNA Stability Provides another Control Mechanism. Contents: Essay on Introduction to Regulation of Gene Expression Essay on the Lac Operon Essay on the Bacteriophage [...]
The below mentioned article provides a short note on gene mutation. Type of Gene Mutation: An alteration in the sequence of bases in a gene due to change, removal or insertion of one or more bases in a gene resulting in an altered gene product is called mutation. (a) Single Base Changes (Point Mutations): It may be transitions or trans-versions. [...]