In this article we will discuss about:- 1. Meaning of Mutations 2. Occurrence of Mutations 3. Expression. Meaning of Mutations: Inheritance is based on genes that are faithfully transmitted from generation to generation in microorganisms (in all organisms). Despite the biochemical mechanisms that facilitate such transmissions faithfully, sudden change in the sequence of nucleotide bases in genes can and do [...]
The following points highlight the top four examples to substantiate the practical implacability of microbial mutants. 1. Induced mutants have been used extensively in the investigation of various biochemical processes, particularly metabolic reactions. Metabolic processes occur via sequences of enzyme-catalyzed reactions and by developing mutants in which the genes coding for enzymes are mutated (altered), the sequence of steps in [...]
The following points highlight the top four techniques used for the isolation of microbial mutants. The techniques are: 1. Direct Observation 2. Enrichment Technique 3. Replica-Plating Technique 4. The Ames Test. 1. Direct Observation Technique: In some cases, a colony growing on an agar plate can easily be seen to be different from the normal parental type (wild-type). For example, [...]