The following points highlight the four methods to detect and isolate mutants. The methods are: 1. Replica Plating Technique 2. Resistance Selection Method 3. Substrate Utilization Method 4. Carcinogenicity Test. 1. Replica Plating Technique: Lederberg and Lederberg (1952) have given replica plating technique. This technique is used to detect auxotrophic mutants which differentiates between mutants and wild type strains on [...]
In this article we will discuss about:- 1. Definition of Mutation 2. History of Mutation 3. Aniridia in Man 4. Detection 5. Types. Contents: Definition of Mutation History of Mutation Aniridia in Man as a Case of Mutation Detection of Mutation Types of Mutation 1. Definition of Mutation: Heredity results accurate reproduction of genes. During cell-division the chromosomes divide to [...]
The following points highlight the eight important types of microbial mutants. The types are: 1. Auxotrophic Mutants 2. Resistant Mutants 3. Cryptic Mutants 4. Conditionally Expressed Mutants 5. Antigenic Mutants 6. Metabolic Mutants 7. Regulatory Mutants 8. Temperature-Sensitive Mutants. Microbial Mutant: Type # 1. Auxotrophic Mutants: Auxotrophic mutants are those that are nutritionally deficient, i.e., they are unable to synthesize [...]