Read this article to learn about the gene libraries, genomic libraries and cDNA libraries. DNA (Gene) Libraries: A DNA library is a set of cloned fragments that collectively represent the genes of a particular organism. Particular genes can be isolated from DNA libraries, much as books can be obtained from conventional libraries. The secret is knowing where and how to [...]
This article throws light upon the top five screening strategies of gene libraries. The top five screening strategies are: (1) Screening by DNA Hybridization (2) Screening by Colony Hybridization (3) Screening by PCR (4) Screening by Immunological Assay and (5) Screening by Protein Function. 1. Screening by DNA Hybridization: The target sequence in a DNA can be determined with a [...]
Read this article to learn about the creation of gene libraries with diagram. Gene Libraries: The collection of DNA fragments (specifically genes) from a particular species represents gene libraries. The creation or construction of gene libraries (broadly genomic libraries) is accomplished by isolating the complete genome (entire DNA from a cell) which is cut into fragments, and cloned in suitable [...]