In this article we will discuss about:- 1. Subject-Matter of Restriction Enzymes 2. Nomenclature of Restriction Enzymes 3. Types 4. Sites 5. Restriction Enzymes Generated Staggered and Blunt Ends 6. Purification. Subject-Matter of Restriction Enzymes: Restriction enzymes, also known as restriction endonucleases, have played a key role in the development of recombinant DNA technology. These have been found in microorganisms [...]
in this article we will discuss about enzymes and its production on commercial scale. Enzyme is an organic catalyst formed by a living cell. Enzymes are mostly obtained from plant and animal cells. Now-a-days microorganisms are becoming excellent sources for industrial production of certain enzymes. Many microorganisms are known to excrete enzymes into their growth media during the fermentation processes [...]
In this article we will discuss about the classification of enzymes. The enzymes are classified by the reactions they catalyze by adding the "ase" to the name of their substrate or to a suffix word or phrase which is meant for their activity, e.g., urease which catalyzes the hydrolysis of urea, and DNA polymerase which catalyzes the synthesis of DNA. [...]