This article throws light upon the seven types of enzymes involved in DNA synthesis and cloning. The seven types of enzymes are: (1) Alkaline Phosphatase (2) Terminal Transferase (3) Thermo-stable DNA Polymerases (4) Bacteriophage RNA Polymerases (5) Nucleases: DNase and RNase (6) Polynucleotide Kinase and (7) DNA Ligase. Type # A. Alkaline Phosphatase: Alkaline phosphatase removes 5' phosphate groups from [...]
This article provides a close look on the DNA Polymerase enzymes. It also describes the role of different types of eukaryotic polymerases in DNA synthesis. DNA Polymerase: The principal chemical reaction catalysed by a DNA polymerase is the 5' 3' synthesis of a DNA polynucleotide. DNA polymerases carry out the process of addition of nucleotides and formation of polynucleotide chain. [...]
This article provides a close look on Enzymology. Enzymes are biocatalysts — the catalysts of life. A catalyst is defined as a substance that increases the velocity or rate of a chemical reaction without itself undergoing any change in the overall process. Enzymes may be defined as biocatalysts synthesized by living cells. They are protein in nature, colloidal and thermolabile [...]