This article throws light upon a common strategy for initiating an enzymatic reaction and learn about how it also controls the running of enzymatic reaction. Initiating, Mixing, and Stopping Reactions: In a typical enzyme assay, all but one of the components of the reaction mixture are added to the reaction vessel, and the reaction is started at time zero by [...]
Read this article to learn about the initial velocity measurement of a chemical reaction by direct, indirect and coupled assays. To measure the velocity of a reaction, it is necessary to follow a signal that reports product formation or substrate depletion over time. The type of signal that is followed varies from assay to assay but usually relies on some [...]
This article clarifies the difference between continuous assays and end point assays used for reaction velocity measurement. Once an appropriate linear time period has been established, the researcher has two options for obtaining a velocity measurement. First, the signal might be monitored at discrete intervals over the entire linear time period, or some convenient portion thereof. This strategy, referred to [...]