Let us make an in-depth study of the enzymes. After reading this article you will learn about: 1. Meaning of Enzymes 2. Classification of Enzymes 3. Mechanism of Enzyme Action 4. Factors Affecting Enzyme Action 5. Enzyme Specificity 6. Enzyme Inhibition and 7. Diagnostic Importance of Enzymes. Meaning of Enzymes: Enzymes are proteinaceous (and even nucleic acids) biocatalyst which alter [...]
In this article, we propose to discuss various experiments on enzymes and enzyme action. A. Hydrolytic Enzymes: I. Amylase (or Diastase): (a) Make thin starch paste by shaking up a little ordinary starch or wheat flour with 50 ml boiling water and standing it to cool. Get some germinating pea seedlings, 2-3 g. (in which the radicle has grown out [...]
This article throws light upon the top five types of methods devised for enzyme kinetics measurement. The top five types of methods are: (1) Detection Methods (2) Assays Based on Optical Spectroscopy (3) Fluorescence Measurements (4) Radio Isotopic Measurements and (5) Other Detection Methods. Method # 1. Detection Methods: A wide variety of physicochemical methods have been used to follow [...]