In this notes we will discuss about:- 1. Nomenclature and Classification of Enzymes 2. Kinetics of Enzyme-Reactions 3. Enzyme Activity and Assay. Notes # Nomenclature and Classification of Enzymes: Before a scientific nomenclature of enzymes was developed, they were given trivial names, like pepsin, trypsin, rennin, ptyaline etc. Later, a more logical system of nomenclature was adopted by adding the [...]
These are endonucleases which cleave double-stranded DNA molecules at specific sites having a sequence of generally four to six nucleotides. These sites are called restriction sites. A specific restriction enzyme cleaves DNA wherever the site occurs. The nucleotide sequence of the restriction site is specific for each of hundreds of restriction enzymes discovered so far from different microorganisms. A restriction [...]
Isozymes: Definition, Occurrence and Characteristics! Definition of Isozymes: The enzymes that occur in a number of different forms and differ from each other chemically, immunologically and electrophoretically are called "Isoenzymes" or "isozymes". Occurrence of Isozymes: Isozymes are present in the serum and tissues of mammals, amphibians, birds, insects, plants and unicellular organisms. Examples: Isozymes of numerous dehydrogenases, and several oxidases, [...]