In this article we will discuss about the steps and applications of Recombinant DNA Technology. Steps Involved in Recombinant DNA Technology: The sequences of steps in r-DNA technology are as follows: (i) Cell culture of cells with the required DNA sequences is obtained through tissue culture technique, (ii) Specific restriction endonuclease is selected from the specific bacterium, (iii) Restriction endonuclease [...]
In this article we will discuss about the top five techniques used for cloning of specific genes and their identification. The techniques are: 1. Direct Selection of Recombinant Clones 2. Colony Hybridization 3. Complementary DNA (c-DNA) 4. Isolation of a Specific Gene: Southern Blotting 5. Polymerase Chain Reaction (PCR). The restriction digest always contains an assortment of donor DNA fragments [...]
In this article we will discuss about the three main types of vectors used in cloning for larger molecules of DNA. The types of vectors are: 1. Plasmid Vectors 2. Bacteriophage Vectors 3. Cosmid Vectors. 1. Plasmid Vectors: Plasmids are the extra chromosomal genetic elements commonly found in bacteria and are mostly made of double-stranded circular DNA. They are used [...]