In this article we will discuss about the meaning an classes of DNA polymorphisms. Meaning of DNA Polymorphisms: Different alleles of a gene produce different phenotypes which can be detected by making crosses between parents with different alleles of two or more genes. Then by determining recombinants in the progeny, a genetic map can be deduced. These are low resolution [...]
The following points highlight the seven main stages of recombinant DNA technology. The stages are: 1. Isolation of the Genetic Material (DNA) 2. Cutting of DNA at Specific Locations 3. Isolation of Desired DNA Fragment 4. Amplification of Gene of Interest using PCR 5. Ligation of DNA Fragment into a Vector 6. Insertion of Recombinant DNA into the Host Cell/Organisms [...]
The following points highlight the four main features that are required to facilitate cloning into a vector. The features are: 1. Origin of Replication 2. Selectable Marker 3. Cloning (Recognition) Sites 4. Vectors for Cloning Genes in Plants and Animals. Feature # 1. Origin of Replication (Ori): This is a sequence from where replication starts and any piece of DNA, [...]